with 80mph being round 3000rpm, changing to the 2.50 ratio would drop revs to 2631rpm @ 80mph
done some quick maths & interestingly the final drive ratio appears to kind of take the tranny gearing (when in 5th) out of the equation. think 5th is around 1:1 ratio anyway
take a Metzeler 240/50 R16 rear tyre. 16" is 406mm, plus the 50 aspect ratio on a 240 section means 120mm tyre depth to rim. 406+120+120 = 646mm overall rear wheel diameter.
circumference C = 2 TT R, so in this case C = 2(22/7)323 in mm
=2030mm
=203cm
1 mile is 1,609.34m or 160,934cm
so the rear wheel must rotate (assuming no slip) 792.78 times per 1 mile
since we are talking about 80mph as the example then the rear must rotate 63,422 times in 1 hour so that's 63,422 RPH @ rear wheel
or 1057 RPM @ rear wheel
now - final drive ratio based on 13/37 is 2.85, so engine is running 3000 RPM, divide that by the 2.85 ratio gives 1052 RPM @ rear wheel
5 RPM difference in results going about it in 2 different ways
change the 2.85 to 2.50 and that should work back to 2631 engine RPM @ 80mph
I'm lazy but found a way to check
some of your maths

. Does anyone know the maximum tyre width and/or aspect ratio that will fit the stock rim and swingarm setup? Maybe better and cheaper to go aftermarket rims like IMFASTOO has to get a wider choice of rear tires.
240/50-R16 vs 240/55-R16 Tire Comparison - Tire Size Calculator | Tacoma World

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240/50-16 240/55-16 Difference
Diameter inches (mm) 25.45 (646.4) 26.39 (670.4) 0.94 (24)
3.7%
Width inches (mm) 9.45 (240) 9.45 (240) 0 (0)
0%
Circum. inches (mm) 79.95 (2030.73) 82.92 (2106.12) 2.97 (75.4)
3.7%
Sidewall Height inches (mm) 4.72 (120) 5.2 (132) 0.47 (12)
10%
Revolutions per mile (km) 792.5 (492.43) 764.13 (474.81) -28.37 (-17.63)
-3.6%
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Speedometer Difference
Speedo Reading Actual Speed
20 mph (32.19 km/h) 20.74 mph (33.38 km/h)
25 mph (40.23 km/h) 25.93 mph (41.73 km/h)
30 mph (48.28 km/h) 31.11 mph (50.07 km/h)
35 mph (56.33 km/h) 36.3 mph (58.42 km/h)
40 mph (64.37 km/h) 41.49 mph (66.76 km/h)
45 mph (72.42 km/h) 46.67 mph (75.11 km/h)
50 mph (80.47 km/h) 51.86 mph (83.46 km/h)
55 mph (88.51 km/h) 57.04 mph (91.8 km/h)
60 mph (96.56 km/h) 62.23 mph (100.15 km/h)
65 mph (104.61 km/h) 67.41 mph (108.49 km/h)
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Gear Ratio / RPMs for New Tire (@ 65 mph)
2.35 2.73 2.94 3.07 3.21 3.31 3.42 3.55 3.73 3.91 4.11 4.27 4.56 4.88 5.13 5.29 5.38 5.71 6.17 7.17
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