Hey. First, I dont know whats been modified on the bike, or what tune you loaded so take what I say with that in mind.
You said "you loaded a tune from tunecu" into the bike. Id guess the previous tune had the o2 box unchecked (no engine light on), and the current tune has the o2 checked (check engine light on)?
Regarding reinstalling the narrow band sensor, id say clean it with a solvent rated for sensors and reinstall if thats what you wish....make sure the tune you loaded has the o2 checked, and run the 12 minute tune and go from there.....